Dutch international Roord, who spent two seasons with the English side before leaving for Germany in 2021, opened the scoring after just nine minutes after Arsenal failed to clear a corner.
Wolfsburg continued to dominate and the unfortunate Lea Williamson put through her own goal in the 73rd minute as she tried to block a dangerous cross from Iceland international Sveindis Jane Jonsdottir.
Victory gave Wolfsburg a 3-1 aggregate win after the first leg of their quarter-final ended 1-1 in London last week.
Later Thursday, seven-time champions Lyon look to overturn a 2-1 first leg deficit against Juventus.
On Wednesday, a world record crowd for a women's football match of 91,553 saw Barcelona cruise into the semi-finals by thrashing Real Madrid 5-2 at Camp Nou on Wednesday to seal an 8-3 win on aggregate.
Paris Saint-Germain needed extra-time to secure a 2-2 home draw with Bayern Munich to progress 4-3 on aggregate.
March 31, 2022