The first leg will take place at Cultural Leonesa's 13,500-seater Reino de León stadium on October 31st, with the return leg scheduled for December 5th at the Camp Nou.
Barcelona landed a draw against a lower-league side by virtue of having qualified for the Champions League last season, with LaLiga clubs only entering the competition at the last-32 stage.
The Catalans won last years iteration of the competition, thumping Sevilla 5-0 in the final at Atletico Madrid's Wanda Metropolitano stadium.
Cultural have beaten Navalcarnero (2-0), Fuenlabrada (1-1 A.E.T. 4-3 pens) and Lorca (3-0) to reach this stage and are one of just five Segunda B sides left in the competition.
Barcelona's defence will have to shackle the top scorer in the competition, with no player having scored more than Cultural's experienced striker Adriane Stantana's tally of three.
October 19, 2018